Chaillet Research Group
Principal Investigator:
Richard Chaillet, PhD, MD
The Chaillet lab uses mouse and ES cell models to study molecular mechanisms of genomic imprinting and epigenetic reprogramming.
Lab Members
Dr. Leonardo D’Aiuto, PhD
Postdoctoral Fellow
daiutol@upmc.edu
Dr. Ewa Borowczyk, PhD
Postdoctoral Fellow
ewa.borowczyk@yahoo.com
Ms. Ashwini Balakrishnan
Graduate Student
asb50@pitt.edu
Mr. Erik Koppes
Graduate Student
eak37@pitt.edu
Selected Publications
- Howell, CY, Bestor, TH, Ding, F, Latham, KE, Mertineit, C, Trasler, JM, & Chaillet, JR. Genomic imprinting disrupted by a maternal-effect mutation in the Dnmt1 gene. Cell, 104:829-38, 2001.
- Ratnam, S, Mertineit, C, Ding, F, Howell, CY, Clarke, HJ, Bestor, TH, Chaillet, JR, & Trasler, JM. Dynamics of Dnmt1 methyltransferase expression and intracellular localization during oogenesis and preimplantation development. Dev Biol, 245:304-14, 2002.
- Ding, F & Chaillet, JR. In vivo stabilization of the Dnmt1 (cytosine-5)-methyltransferase protein. Proc Natl Acad Sci USA, 99:14861-6, 2002.
- Ben-Yehudah, A, Reinhart, B, Navara, C, Kotzuk, J, Witchel, S, Schatten, G, & Chaillet, JR. Specific dynamic and non-invasive labeling of pancreatic b cells in reporter mice. Genesis, 43:166-74, 2005.
- Reinhart, B, Paoloni-Giacobino, A, & Chaillet, JR. Specific differentially methylated domain sequences direct the maintenance of methylation at imprinted genes. Mol Cell Biol, 26:8347-56, 2006.
- Green, K, Lewis, A, Dawson, C, Dean, W, Reinhart, B, Chaillet, JR, & Reik, W. A developmental window of opportunity for imprinted gene silencing mediated by DNA methylation and the Kcnq1ot1 non-coding RNA. Mammalian Genome, 18:32-42, 2007.
- Paoloni-Giacobino, A, D'Aiuto, L, Cirio, MC, Reinhart, B, & Chaillet, JR. Conserved features of imprinted differentially methylated domains. Gene, 399:33-45, 2007.
- Toppings, M, Castro, C, Mills. PH, Reinhart, B, Schatten, G, Ahrens, ET, Chaillet, JR, & Trasler, JM. Profound phenotypic variation among mice deficient in the maintenance of genomic imprints. Hum Reprod, 23:807-18, 2008.
- Cirio MC, Ratnam S, Ding F, Reinhart B, Navara C, & Chaillet JR.Preimplantation expression of the somatic form of Dnmt1 suggests a role in the inheritance of genomic imprints. BMC Dev Biol, 8:9, Doi:10.1186/1471-213X-8-9, 2008.
- D'Aiuto L, Robison CS, Gigante M, Nwanegbo E, Shaffer B, Sukhwani M, Castro CA, & Chaillet JR. Human IL-12 p40 as a reporter gene for high-throughput screening of engineered mouse embryonic stem cells. BMC Biotechnol, 3;8:52, 2008.
- Cirio MC, Martel J, Mann M, Toppings M, Bartolomei M, Trasler J, & Chaillet JR. DNA methyltransferase 1o functions during preimplantation development to preclude a profound level of epigenetic variation. Dev Biol, 324:139-50, 2008.
- Ben-Yehudah A, White C, Navara CS, Castro CA, Ize-Ludlow D, Shaffer B, Sukhwani M, Mathews CE, Chaillet JR, & Witchel SF. Evaluating protocols for embryonic stem cell differentiation into insulin secreting b-cells using Insulin II-GFP as a specific and non-invasive reporter. Cloning Stem Cells, 11:245-57, 2009.
- Borowczyk E, Mohan KN, D’Aiuto L, Cirio MC, & Chaillet JR. Identification of a region of the DNMT1 methyltransferase that regulates the maintenance of genomic imprints. Proc Natl Acad Sci USA, 2009 (in press).